HLA-DPA1/HLA-DPB1

利用同源重组，将小鼠H2-Aa和H2-Ab1基因进行人源化修饰。

Fig.1 Detection of HLA-DPA1/HLA-DPB1 expression in large intestine and spleen by RT-PCR. 

Wild type: one band at 278 bp with primers F4/R4(mH2-Aa) and one band at 123 bp with primers F/R(mGapdh);

Heterozygous: one band at 278 bp with primers F4/R4(mH2-Aa) and one band at 107 bp with primers F1/R1(hHLA-DPA1) and one band at 123 bp with primers F/R(mGapdh).

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of HLA-DPA1/HLA-DPB1 expression in large intestine and spleen by RT-PCR. 

Wild type: one band at 299 bp with primers F3/R3(mH2-Ab1) and one band at 123 bp with primers F/R(mGapdh);

Heterozygous: one band at 299 bp with primers F3/R3(mH2-Ab1), one band at 113 bp with primers F2/R2(hHLA-DPB1) and one band at 123 bp with primers F/R(mGapdh).

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

Fig.3 Detection of MHC-Ⅱ expression on B cells and DCs in Peripheral blood in 13-week-old WT C57BL/6 and HE HLA-DPA1/HLA-DPB1 mice.

Fig.4 Detection of MHC-Ⅱ expression on B cells and DCs in Spleen in 13-week-old WT C57BL/6 and HE HLA-DPA1/HLA-DPB1 mice.