淀粉样前体蛋白APP与AD有着密切的联系，其分解产生的B-淀粉样蛋AB在脑中的堆积是导致阿尔茨海默病的重要因素。南模生物通过ES细胞打靶技术，将小鼠App基因部分替换为人源APPNL-G-F，从而表达人鼠嵌合APPNL-G-F蛋白，该纯合子小鼠随着年龄增长，大脑会逐渐出现明显的斑块累积症状。
Fig1. Detection of APP expression in brain by RT-PCR.
Fig2. (A) β-Amyloid (6E10) staining of WT and hAPPNL-G-F mice at 1, 3 and 6 months (magnification, 40x and 100x). (B) Statistics of 6E10 positive plaque area.
Fig3. (A) Thioflavine S staining of WT and hAPPNL-G-F mice at 1, 3 and 6 months (magnification, 40x and 100x). (B) Statistics of ThiS positive plaque area.
Mutations: APP K670_M671delinsNL (Swedish), APP I716V (Florida), APP V717I (London), PSEN1 M146L (A>C), PSEN1 L286V
Fig 1. Expression of Aβ in brain of 6-month-old 5xFAD mice.
Fig 2. ELISA analysis of CSF sample collected from 5xFAD mice for human Aβ42.
Fig 3. Results of Aβ 6E10 and Iba1 immunocostaining in 5xFAD mice after 3 months of Lecanemab treatment (90mpk, Q2W; n=6, male; dosing started at 7w).
Fig 4. Aβ 6E10 Area fraction in cortex and hippocampis of 5xFAD mice after Lecanemab treatment.
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