R26-rtTA/TIGRE-pTRE-hDMPK(exon 11-15)

Fig.1 Detection of hDMPK(exon 11-15)-3'UTR(CTG)200-DMPK  expression in muscle and heart by qPCR. 

Each value in G1 and G2 represents the mRNA level relative to the value in G3(n=3).

Abbr. WT, wild type. Doxy, Doxycycline.

Fig.2 Body weight growth curve of R26-rtTA-TIGRE-DM200 (Doxy) mice. Doxy administration start from 6-8 weeks old.

Abbr. WT, Wild type; Doxy, Doxycycline.

Fig.3 8 weeks post doxy induction, (A-C) significant skeletal muscle wasting is observed in gastrocnemius and tibialis anterior (TA) muscles from R26-rtTA-TIGRE-DM200 (Doxy) mice. (D) Tibia length is unaffected in R26-rtTA-TIGRE-DM200 (Doxy) mice compared with C57BL/6. (E) Representative image of detection of muscle loss(arrows). (n=3). *P<0.05, **P<0.01, ***P<0.001

Abbr. WT, Wild type; Doxy, Doxycycline.

Fig.4 Histopathology of C57BL6 and R26-rtTA-TIGRE-DM200 (Doxy) mice at 8 weeks post doxy induction. (A) H&E staining images of Gastrocnemius. (B) Fiber CSA (143 fibers per sample). Quantified in (C). (n=3). 

Abbr. WT, Wild type; Doxy, Doxycycline; CSA, Cross Sectional Area.