hIL4-Tg(M-NSG)

利用转基因技术将人源IL4基因表达框整合到小鼠基因组中，从而表达人源IL4。

Fig 1. Detection of IL4 expression in adrenal, intestine and lung of 8-week-old male mice by RT-PCR. 

Wild type: Only one band at 123 bp with primers F/R(mGapdh).

Homozygous: One band at 223 bp with primers F1/R1(hIL4) and one band at 123 bp with primers F/R(mGapdh).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig 2. Detection of IL4 expression in adrenal, intestine and lung of 8-week-old male mice by qPCR (n=2). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig 3. Detection of human IL4 expression in serum of 8-week-old male mice by ELISA (n=2). 

Abbr.  HO, homozygous; WT, wild type; N.D. not detected.

Fig 4. Body weight growth curve of huPBMC-M-NSG-hIL4 mice. 

Fig 4. Survival rate of huPBMC-M-NSG-hIL4 mice. 

Fig 5. GvHD scores of huPBMC-M-NSG-hIL4 mice. 

Fig 6. Percentage of hCD45-positive cells in peripheral blood of huPBMC-M-NSG-hIL4 mice. 

Fig 7. Flow analysis of peripheral blood lymphocyte subpopulations in the huPBMC-M-NSG-hIL4 model. Human PBMCs (1E7) were injected intravenously into hIL4-Tg(M-NSG) mice (female, 6-8 weeks old, n=10). Blood from mice at different time points after implantation of human PBMCs was taken for flow cytometric analysis.