hAPP(3)

Fig.1 Detection of APP expression in cerebellum, cortex, frontal lobe and lung by RT-PCR. Cerebellum, cortex, frontal lobe and lung RNA were extracted from 12-year-old male wild-type C57BL/6 (WT)(n=3) and heterozygous hAPP(3) knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse App mRNA (319 bp) was detectable in both wild-type C57BL/6 mice and heterozygous hAPP(3) knockin mice. Human APP mRNA (282 bp) was detectable only in heterozygous hAPP(3) knockin mice but not in wild-type mice.

Abbr. M, marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of APP expression in hAPP(3) mice by WB. The hippocampus, cortex, liver and cerebellum tissue lysates were collected from 6-week-old male wild-type C57BL/6 mice and 6-week-old male heterozygous hAPP(3) mice, and then analyzed by western blot with anti-APP antibody. APP was detectable in hippocampus, cortex, liver and cerebellum from both WT C57BL/6 and HE hAPP(3) mice , as the antibody was cross-reactived between human and mouse APP. 

Abbr. HE, heterozygous; WT, wild type; PC, positive control, Hela cells.