Lat-Y136F

品系全名

C57BL/6JSmo-Latem1(Y136F）Smoc

目录号

NM-KI-200249

品系状态

活体

导出PDF

基因信息

基因名
Lat

基因曾用名

pp36; p36-38

染色体位置

7

NCBI ID

16797

MGI ID

1342293

Ensembl ID

ENSMUSG00000030742

Pubmed

Lat

人类同源基因

LAT

品系描述

将Lat中第136位氨基酸由Y变为F，建立Lat点突变小鼠模型。
应用领域：罕见病模型--IgG4相关性疾病

疾病预测

IgG4相关性疾病
Igg4-Related Disease

近似模型的表型

MGI:2385971

参考文献

Aguado E, et al., Induction of T helper type 2 immunity by a point mutation in the LAT adaptor. Science. 2002 Jun 14;296(5575):2036-40

验证数据

image.png

Fig 1. Spleen (left) and mesenteric lymph nodes (right) from 8-week-old wild-type and Lat-Y136F mice.

image.png

Fig 2. Flow cytometry analysis of different subsets of T cells across tissues in homozygous Lat-Y136F mice in mesenteric lymph nodes (MLN) (n=3).

image.png

Fig 3. Flow cytometry analysis of different subsets of T cells across tissues in homozygous Lat-Y136F mice in peripheral blood (n=3).

image.png

Fig 4. Flow cytometry analysis of different subsets of T cells across tissues in homozygous Lat-Y136F mice in spleen (n=3).

image.png

Fig 5. Flow cytometry analysis of different subsets of T cells across tissues in homozygous Lat-Y136F mice in thymus (n=3).

image.png

Fig 6. Serum IgG1 and IgE levels in homozygous Lat-Y136F mice (n=3-5).

image.png

Fig 7. Histopathological analysis across organs in homozygous Lat-Y136F mice(n=3-4). Scale bar=100 μm; magnification,  200×.

image.png

Fig 8. Serum IgG1 level and body weight post 3 weeks treatment in 4/7-week old homozygous Lat-Y136F mice (n=5).

image.png

Fig 9. Wet weight and photos of spleen and mesenteric lymph nodes (MLN) upon prednisolone treatment in 7-week old homozygous Lat-Y136F mice (n=5).

image.png

Fig. 10 Histopathology changes upon prednisolone treatment. Representative histopathological appearance of kidney and pancreas in 7-week-old prednisolone-treated and saline-treated control mice. Red arrows indicate inflammatory lesions and black arrows indicate fibrosis in the kidney and pancreas of C57 control mice, saline- or prednisolone-treated Lat-Y136F mice (n=5). Data are presented as mean and ± SEM. Scale bar=100 μm; magnification, 200×.

你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

查看

【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

查看

Cre-lox系统介绍及使用汇总

你一定听说过Cre-lox重组系统，无论你是否直接进行过基因操作。由于Cre-lox系统具有操作简单、重组率高的优点，如今已经成为体内外遗传操作的强有力工具。利用Cre-lox系统，可以在特定细胞、组织或整个生物体，甚至在特定时间点敲除或表达某个基因，实现对特定基因的时空特异性操作，这对基因功能的研究和人类疾病动物模型的建立都具有深刻影响。

查看