hADIPOQ

Fig.1 Detection of ADIPOQ expression in mammary gland by RT-PCR. Mouse Adipoq mRNA (317 bp) was detectable only in wild-type C57BL/6 mice but not in hADIPOQ mice. Human ADIPOQ mRNA (265 bp) was detectable only in homozygous hADIPOQ mice but not in wild-type mice. Mammary gland RNA was extracted from 8-year-old male WT C57BL/6 and homozygous hADIPOQ knockin mice (n=3 in each group), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human ADIPOQ(A) and mouse ADIPOQ(B) expression in plasma of WT C57BL/6 mice and HO hADIPOQ mice by ELISA. ADIPOQ protein was detectable in plasma from both WT C57BL/6 mice and homozygous hADIPOQ mice. Human ADIPOQ was exclusively detectable in homozygous hADIPOQ mice, while mouse ADIPOQ was exclusively detectable in WT C57BL/6 mice. Plasma was collected from wild-type C57BL/6 mice (n=3, male, 8 weeks old) and HO hDIPOQ mice (n=3, male, 8 weeks old), and analyzed by ELISA with anti-mADIPOQ ELISA kit and anti-hADIPOQ ELISA kit. 

Abbr.  HO, homozygous; WT, wild type.