hF11(3)

Fig.1 Detection of F11 expression in liver by RT-PCR. Mouse F11 mRNA (204 bp) was detectable only in wild-type C57BL/6 mice. Human F11 mRNA (316 bp) was detectable only in homozygous hF11(3) knockin mice but not in wild-type mice. Liver RNA was extracted from 9-year-old male wild-type C57BL/6 (WT) and homozygous hF11(3) knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human F11(A) expression in serum of WT C57BL/6 mice and HO hF11(3) mice by ELISA. F11 was detectable in serum from both WT C57BL/6 and HO hF11(3) mice, Human F11 was exclusively detectable in homozygous hF11(3) knockin mice. Serum was collected from wild-type C57BL/6 mice (n=2, 6 weeks old) and HO hF11(3) mice (n=3, male, 6 weeks old), and analyzed by ELISA with anti-hF11 ELISA kit. 

Abbr.  HO, homozygous; WT, wild type.